Ion-pair vortex assisted liquid-phase microextraction coupled with UV-visible spectrophotometry for the determination of mesalazine in pharmaceutical formulations

Abstract
This study demonstrates an effective, simple, and selective method for monitoring mesalazine in pharmaceutical formulations using liquid-phase microextraction (LPME) and spectrophotometry. Combining LPME with spectrophotometry is an efficient method for analysing various compounds in different matrices. This method is based on extracting the ion pair formed between the blue indophenol produced by the oxidative reaction of mesalazine and syringic acid in an alkaline medium and a quaternary ammonium salt into a microvolume of organic solvent. The experimental parameters influencing LPME performance, such as the type and concentration of the quaternary ammonium ion salt and the type and volume of the extractant solvent, were optimised for optimal detection. The linear range and the limit of detection for measuring red species in pharmaceutical formulations were determined to be 0.005–0.080 μg mL−1 and 0.003 μg mL−1, respectively, with a relative standard deviation of 4%–6%. The method had a preconcentration factor of 50 at 520 nm, making it highly efficient and reliable for monitoring mesalazine in pharmaceutical formulations.\r\n\r\n

Author
Diyar Salahuddin Ali

DOI
https://doi.org/10.1016/j.pharma.2023.10.011

Publisher
Annales Pharmaceutiques Françaises

ISSN
2772-803X

Publish Date: